human PKM2 shRNA silencing Adenovirus

Name
Ad-h-PKM2-shRNA

Cat No
shADV-219036

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PKM.

shADV-219036

Ad-h-PKM2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PKM (pyruvate kinase, muscle). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

CTHBP; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; HEL-S-30; OIP3; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; PK3; PKM2; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase PKM; pyruvate kinase, muscle; TCB; THBP1; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK

Description (eCommerce)

Pyruvate Kinase M2 (PKM2) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a general role in caspase independent cell death of tumor cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P14618].

Description (Vector)

PKM encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Gene ID

5315

Gene Name (eCommerce)

pyruvate kinase M1/2

Gene Name (Vector)

pyruvate kinase, muscle

Gene Symbol

PKM

HGNC ID

HGNC:9021

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1101

ORF Size (bp)

1101 bp

Protein Name (eCommerce)

Pyruvate kinase PKM

RefSeq ID

NM_182471

RefSeq Synonyms

NM_182471, NM_182470, NM_002654, NM_001316318, NM_001206799, NM_001206798, NM_001206797, NM_001206796, BC094767, BC035198, BC012811, BC007952, BC007640, BC000481,

Research Areas

Apoptosis,Cancer,EGFR Signaling,Host-Virus Interactions,Hypoxia,Immunology,Metabolism/Metabolic Process,Mitochondrion,Signal Transduction,T-cell Receptor Signaling,Viral Carcinogenesis

Research Areas (Faceted)

cancer,signal_transduction,cell_biology,infectious_disease,cell_biology,metabolism,immunology,metabolism,cell_biology,signal_transduction,immunology,cancer,infectious_disease

Species

human

Target Sentence

UniGene ID

Hs.534770

UniProt ID (eCommerce)

P14618

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.