human PIKFYVE shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human PIKFYVE.
shADV-218966
Ad-h-PIKFYVE-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human PIKFYVE (phosphoinositide kinase, FYVE-type zinc finger containing). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
Description (Vector)
Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
200576
Gene Name (Vector)
phosphoinositide kinase, FYVE-type zinc finger containing
Gene Symbol
PIKFYVE
HGNC ID
HGNC:23785
ORF Size (aa)
6294
ORF Size (bp)
6294 bp
RefSeq ID
NM_015040
RefSeq Synonyms
NM_152671, NM_015040, NM_001178000, BC172527, BC156451, BC032389,
Species
human
UniGene ID
Hs.173939
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.