human PFKP shRNA silencing Adenovirus

Name 
Ad-h-PFKP-shRNA
Cat No 
shADV-218688
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PFKP.

shADV-218688
Ad-h-PFKP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PFKP (phosphofructokinase, platelet). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-C; PFKF; PFK-P; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase
Description (eCommerce)
Phosphofructokinase 1 catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q01813].
Description (Vector)
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) (PubMed 6451249) determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.(supplied by OMIM, Mar 2008)
Gene ID
5214
Gene Name (eCommerce)
phosphofructokinase, platelet
Gene Name (Vector)
phosphofructokinase, platelet
Gene Symbol
PFKP
HGNC ID
HGNC:8878
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2355
ORF Size (bp)
2355 bp
Protein Name (eCommerce)
ATP-dependent 6-phosphofructokinase, platelet type
RefSeq ID
NM_002627
RefSeq Synonyms
NM_002627, NM_001345944, NM_001323074, NM_001323073, NM_001323072, NM_001323071, NM_001323070, NM_001323069, NM_001323068, NM_001323067, NM_001242339, BC029138, BC002536,
Research Areas
Metabolism/Metabolic Process
Research Areas (Faceted)
cell_biology,metabolism
Species
human
Target Sentence
Phosphofructokinase 1 catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q01813].
UniGene ID
Hs.26010
UniProt ID (eCommerce)
Q01813

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.