human PFKL shRNA silencing Adenovirus

Name 
Ad-h-PFKL-shRNA
Cat No 
shADV-218683
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PFKL.

shADV-218683
Ad-h-PFKL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PFKL (phosphofructokinase, liver type). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; liver-type 1-phosphofructokinase; PFK-B; PFK-L; phosphofructo-1-kinase isozyme B; phosphohexokinase
Description (eCommerce)
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P17858].
Description (Vector)
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).
Gene ID
5211
Gene Name (eCommerce)
phosphofructokinase, liver type
Gene Name (Vector)
phosphofructokinase, liver type
Gene Symbol
PFKL
HGNC ID
HGNC:8876
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2484
ORF Size (bp)
2484 bp
Protein Name (eCommerce)
ATP-dependent 6-phosphofructokinase, liver type
RefSeq ID
NM_002626
RefSeq Synonyms
NM_002626, NM_001002021, BC009919, BC008964, BC007536, BC006422,
Research Areas
HIF-1 Signaling,Insulin Signaling,Kinase/Phosphatase,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
signal_transduction,metabolism,cell_biology
Species
human
Target Sentence
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P17858].
UniGene ID
Hs.255093
UniProt ID (eCommerce)
P17858

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.