human PDK3 shRNA silencing Adenovirus

Name 
Ad-h-PDK3-shRNA
Cat No 
shADV-218538
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PDK3.

shADV-218538
Ad-h-PDK3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PDK3 (pyruvate dehydrogenase kinase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CMTX6; GS1-358P8.4
Description (Vector)
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
5165
Gene Name (Vector)
pyruvate dehydrogenase kinase 3
Gene Symbol
PDK3
HGNC ID
HGNC:8811
ORF Size (aa)
1221
ORF Size (bp)
1221 bp
RefSeq ID
NM_005391
RefSeq Synonyms
NM_005391, NM_001142386, BC015948,
Species
human
UniGene ID
Hs.658190

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.