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human PC shRNA silencing Adenovirus

Name 
Ad-h-PC-shRNA
Cat No 
shADV-218303
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PC.

shADV-218303
Ad-h-PC-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PC (pyruvate carboxylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
96
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
PCB
Description (Vector)
PC encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Gene ID
5091
Gene Name (Vector)
pyruvate carboxylase
Gene Symbol
PC
HGNC ID
HGNC:8636
ORF Size (aa)
3537
ORF Size (bp)
3537 bp
RefSeq ID
NM_000920
RefSeq Synonyms
NM_022172, NM_001040716, NM_000920, BC011617,
Species
human
UniGene ID
Hs.89890

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.