human ARFGAP3 shRNA silencing Adenovirus

Name 
Ad-h-ARFGAP3-shRNA
Cat No 
shADV-218109
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ARFGAP3.

shADV-218109
Ad-h-ARFGAP3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ARFGAP3 (ADP ribosylation factor GTPase activating protein 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ADP-ribosylation factor GTPase activating protein 1; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFGAP1
Description (eCommerce)
ADP-ribosylation factor GTPase activating protein 3 (ArfGAP3) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. Although they are differentially regulated, ArfGAP3 along with ArfGAP1 and ArfGAP2 has been shown to play an overlapping role in COPI-mediated transport and in the maintenance of Golgi organization.
Description (Vector)
ARFGAP3 is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
Gene ID
26286
Gene Name (eCommerce)
ADP ribosylation factor GTPase activating protein 3
Gene Name (Vector)
ADP ribosylation factor GTPase activating protein 3
Gene Symbol
ARFGAP3
HGNC ID
HGNC:661
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1551
ORF Size (bp)
1551 bp
Protein Name (eCommerce)
ADP-ribosylation factor GTPase-activating protein 3
RefSeq ID
NM_014570
RefSeq Synonyms
NM_014570, NM_001142293, BC005122,
Research Areas
Golgi Apparatus,Immunology,RAC1 Signaling,Rho Signaling,Signal Transduction,T-cell Receptor Signaling,Transport,Zinc-finger
Research Areas (Faceted)
cell_biology,immunology,signal_transduction
Species
human
Target Sentence
ADP-ribosylation factor GTPase activating protein 3 (ArfGAP3) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. Although they are differentially regulated, ArfGAP3 along with ArfGAP1 and ArfGAP2 has been shown to play an overlapping role in COPI-mediated transport and in the maintenance of Golgi organization.
UniGene ID
Hs.162877
UniProt ID (eCommerce)
Q9NP61

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.