human NSL1 shRNA silencing Adenovirus

Name 
Ad-h-NSL1-shRNA
Cat No 
shADV-217114
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NSL1.

shADV-217114
Ad-h-NSL1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NSL1 (NSL1, MIS12 kinetochore complex component). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C1orf48; DC8; kinetochore-associated protein NSL1 homolog; MIS14; NSL1, MIND kinetochore complex component, homolog
Description (eCommerce)
DC8 is a component of the MIS12 complex which includes DC8, MIS12, DSN1, and PMF1. The MIS12 complex localizes at inner kinetochores to perform an essential function in kinetochore assembly which allows proper chromosome alignment and cell cycle progression.
Description (Vector)
NSL1 encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
25936
Gene Name (eCommerce)
NSL1, MIS12 kinetochore complex component
Gene Name (Vector)
NSL1, MIS12 kinetochore complex component
Gene Symbol
NSL1
HGNC ID
HGNC:24548
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
846
Protein Name (eCommerce)
Kinetochore-associated protein NSL1 homolog
RefSeq ID
NM_015471
RefSeq Synonyms
NM_015471, NM_001297739, NM_001297737, NM_001297736, NM_001042549, BC007067,
Research Areas
Cell Cycle,Cell Division
Research Areas (Faceted)
cell_biology,cell_cycle,genetics
Species
human
Target Sentence
DC8 is a component of the MIS12 complex which includes DC8, MIS12, DSN1, and PMF1. The MIS12 complex localizes at inner kinetochores to perform an essential function in kinetochore assembly which allows proper chromosome alignment and cell cycle progression.
UniGene ID
Hs.497692
UniProt ID (eCommerce)
Q96IY1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.