human NRAS shRNA silencing Adenovirus

Name 
Ad-h-NRAS-shRNA
Cat No 
shADV-217066
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NRAS.

shADV-217066
Ad-h-NRAS-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NRAS (neuroblastoma RAS viral oncogene homolog). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ALPS4;CMNS;GTPase NRas;NCMS;neuroblastoma RAS viral (v-ras) oncogene homolog;neuroblastoma RAS viral oncogene homolog;N-ras;N-ras protein part 4;NRAS1;NS6;transforming protein N-Ras;v-ras neuroblastoma RAS viral oncogene homolog
Description (eCommerce)
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. N-Ras plays an important role in the regulation of cell proliferation and plays a role in promoting oncogenic events and mutations in N-Ras have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [taken from NCBI Entrez Gene (Gene ID: 4893)].
Description (Vector)
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Gene ID
4893
Gene Name (eCommerce)
NRAS proto-oncogene, GTPase
Gene Name (Vector)
neuroblastoma RAS viral oncogene homolog
Gene Symbol
NRAS
HGNC ID
HGNC:7989
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
570
Protein Name (eCommerce)
GTPase NRas
RefSeq ID
NM_002524
RefSeq Synonyms
NM_002524, BC005219,
Research Areas
cell_biology,neurobiology,immunology,signal_transduction,cancer,cardiovascular,infectious_disease,stem_cell_biology
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,immunology,infectious_disease,neurobiology,signal_transduction,stem_cell_biology
Species
human
Target Sentence
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. N-Ras plays an important role in the regulation of cell proliferation and plays a role in promoting oncogenic events and mutations in N-Ras have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [taken from NCBI Entrez Gene (Gene ID: 4893)].
UniGene ID
Hs.486502
UniProt ID (eCommerce)
P01111

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.