human NPHS2 shRNA silencing Adenovirus

Name
Ad-h-NPHS2-shRNA

Cat No
shADV-216963

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human NPHS2.

shADV-216963

Ad-h-NPHS2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NPHS2 (NPHS2, podocin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

PDCN; SRN1

Description (Vector)

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined.

Gene ID

7827

Gene Name (Vector)

NPHS2, podocin

Gene Symbol

NPHS2

HGNC ID

HGNC:13394

ORF Size (aa)

948

ORF Size (bp)

948 bp

RefSeq ID

NM_014625

RefSeq Synonyms

NM_014625, NM_001297575, BC029141,

Species

human

UniGene ID

Hs.412710

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.