human NPC1L1 shRNA silencing Adenovirus

Name
Ad-h-NPC1L1-shRNA

Cat No
shADV-216949

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human NPC1L1.

shADV-216949

Ad-h-NPC1L1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NPC1L1 (NPC1 like intracellular cholesterol transporter 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

NPC11L1

Description (Vector)

NPC1L1 is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

29881

Gene Name (Vector)

NPC1 like intracellular cholesterol transporter 1

Gene Symbol

NPC1L1

HGNC ID

HGNC:7898

ORF Size (aa)

3861

ORF Size (bp)

3861 bp

RefSeq ID

NM_013389

RefSeq Synonyms

NM_013389, NM_001300967, NM_001101648, BC143756, BC117178,

Species

human

UniGene ID

Hs.567486

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.