human NEK2 shRNA silencing Adenovirus

Name 
Ad-h-NEK2-shRNA
Cat No 
shADV-216570
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human NEK2.

shADV-216570
Ad-h-NEK2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NEK2 (NIMA related kinase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HsPK21; NEK2A; NLK1; PPP1R111; RP67
Description (Vector)
NEK2 encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Gene ID
4751
Gene Name (Vector)
NIMA related kinase 2
Gene Symbol
NEK2
HGNC ID
HGNC:7745
ORF Size (aa)
1338
ORF Size (bp)
1338 bp
RefSeq ID
NM_002497
RefSeq Synonyms
NM_002497, NM_001204183, NM_001204182, BC065932, BC043502,
Species
human
UniGene ID
Hs.153704

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.