human NCSTN shRNA silencing Adenovirus

Name 
Ad-h-NCSTN-shRNA
Cat No 
shADV-216441
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human NCSTN.

shADV-216441
Ad-h-NCSTN-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human NCSTN (nicastrin). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
anterior pharynx-defective 2; ATAG1874; nicastrin
Description (eCommerce)
Nicastrin is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92542].
Description (Vector)
NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined.
Gene ID
23385
Gene Name (eCommerce)
nicastrin
Gene Name (Vector)
nicastrin
Gene Symbol
NCSTN
HGNC ID
HGNC:17091
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2070
ORF Size (bp)
2070 bp
Protein Name (eCommerce)
Nicastrin
RefSeq ID
NM_015331
RefSeq Synonyms
NM_015331, NM_001290186, NM_001290184, BC047621,
Research Areas
Alzheimer's Disease,Apoptosis,Cancer,Golgi Apparatus,Immunology,Neurobiology,Neurodegeneration,NGF Signaling,Notch Signaling,Signal Transduction,Toll-like Receptor Signaling,Wnt Signaling
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
Nicastrin is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92542].
UniGene ID
Hs.517249
UniProt ID (eCommerce)
Q92542

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.