human MRPL12 shRNA silencing Adenovirus

Name 
Ad-h-MRPL12-shRNA
Cat No 
shADV-215779
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MRPL12.

shADV-215779
Ad-h-MRPL12-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MRPL12 (mitochondrial ribosomal protein L12). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
5c5-2; L12mt; MRP-L31/34; MRPL7; MRPL7/L12; RPML12
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Gene ID
6182
Gene Name (Vector)
mitochondrial ribosomal protein L12
Gene Symbol
MRPL12
HGNC ID
HGNC:10378
ORF Size (aa)
597
RefSeq ID
NM_002949
RefSeq Synonyms
NM_002949, BC007497, BC002344,
Species
human
UniGene ID
Hs.109059

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.