human MED13 shRNA silencing Adenovirus

Name 
Ad-h-MED13-shRNA
Cat No 
shADV-215237
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human MED13.

shADV-215237
Ad-h-MED13-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MED13 (mediator complex subunit 13). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
activator-recruited cofactor 250 kDa component; ARC250; DRIP250; HSPC221; Mediator complex subunit 13; mediator of RNA polymerase II transcription subunit 13; mediator of RNA polymerase II transcription, subunit 13 homolog; THRAP1; thyroid hormone receptor-associated protein 1; thyroid hormone receptor-associated protein complex 240 kDa component; thyroid hormone receptor-associated protein complex component TRAP240; thyroid hormone receptor-associated protein, 240 kDa subunit; TRAP240; vitamin D3 receptor-interacting protein complex component DRIP250
Description (eCommerce)
THRAP/TRAP240 is a component of the TRAP (thyroid hormone receptor associated protein) complex. The TRAP complex is also known as Mediator and functions as a coactivator of transcription by bridging DNA bound transcription factors to the basal transcription machinery. THRAP1/TRAP240 has been reported to form a complex with MED12, cyclin C, and CDK8 and negatively regulates transcription.
Description (Vector)
MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.
Gene ID
9969
Gene Name (eCommerce)
mediator complex subunit 13
Gene Name (Vector)
mediator complex subunit 13
Gene Symbol
MED13
HGNC ID
HGNC:22474
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
6525
ORF Size (bp)
6525 bp
Protein Name (eCommerce)
Mediator of RNA polymerase II transcription subunit 13
RefSeq ID
NM_005121
RefSeq Synonyms
NM_005121, BC140891,
Research Areas
Gene Expression,Metabolism/Metabolic Process,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,metabolism,transcription_translation
Species
human
Target Sentence
THRAP/TRAP240 is a component of the TRAP (thyroid hormone receptor associated protein) complex. The TRAP complex is also known as Mediator and functions as a coactivator of transcription by bridging DNA bound transcription factors to the basal transcription machinery. THRAP1/TRAP240 has been reported to form a complex with MED12, cyclin C, and CDK8 and negatively regulates transcription.
UniGene ID
Hs.282678
UniProt ID (eCommerce)
Q9UHV7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.