human MCM10 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human MCM10.
shADV-215174
Ad-h-MCM10-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human MCM10 (minichromosome maintenance 10 replication initiation factor). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CNA43; DNA43; homolog of yeast MCM10; hsMCM10; MCM10 minichromosome maintenance deficient 10; minichromosome maintenance complex component 10; PRO2249; protein MCM10 homolog
Description (eCommerce)
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM10 (Mini-Chromosome Maintenance 10) is not a member of the canonical six that form the MCM complex. MCM10 appears to interact with all members of the MCM complex and functions as a factor that recruits the MCM complex to the pre-replication complex (pre-RC) on DNA.
Description (Vector)
MCM10 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Gene ID
55388
Gene Name (eCommerce)
minichromosome maintenance 10 replication initiation factor
Gene Name (Vector)
minichromosome maintenance 10 replication initiation factor
Gene Symbol
MCM10
HGNC ID
HGNC:18043
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2625
ORF Size (bp)
2625 bp
Protein Name (eCommerce)
Protein MCM10 homolog
RefSeq ID
NM_182751
RefSeq Synonyms
NM_182751, NM_018518, BC143490, BC101727, BC009108,
Research Areas
Cell Cycle,DNA Replication,Replication,Zinc-finger
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM10 (Mini-Chromosome Maintenance 10) is not a member of the canonical six that form the MCM complex. MCM10 appears to interact with all members of the MCM complex and functions as a factor that recruits the MCM complex to the pre-replication complex (pre-RC) on DNA.
UniGene ID
Hs.198363
UniProt ID (eCommerce)
Q7L590
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.