human MAP4K4 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human MAP4K4.
shADV-214953
Ad-h-MAP4K4-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human MAP4K4 (mitogen-activated protein kinase kinase kinase kinase 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
epididymis secretory protein Li 31; FLH21957; HEL-S-31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; nck-interacting kinase; NIK; Ste20 group protein kinase HGK
Description (eCommerce)
MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.
Description (Vector)
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Gene ID
9448
Gene Name (eCommerce)
mitogen-activated protein kinase kinase kinase kinase 4
Gene Name (Vector)
mitogen-activated protein kinase kinase kinase kinase 4
Gene Symbol
MAP4K4
HGNC ID
HGNC:6866
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3960
ORF Size (bp)
3960 bp
Protein Name (eCommerce)
Mitogen-activated protein kinase kinase kinase kinase 4
RefSeq ID
NM_145687
RefSeq Synonyms
NM_145687, NM_145686, NM_004834, NM_001242560, NM_001242559, BC156887, BC156089,
Research Areas
CD40 Signaling,Gene Expression,Host-Virus Interactions,Immunology,Insulin Signaling,Interleukin Signaling,JNK Signaling,Kinase/Phosphatase,MAPK Signaling,NF-kB Signaling,Signal Transduction,TNF Signaling
Research Areas (Faceted)
cell_biology,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.
UniGene ID
Hs.431550
UniProt ID (eCommerce)
O95819
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.