human MANEA shRNA silencing Adenovirus

Name
Ad-h-MANEA-shRNA

Cat No
shADV-214892

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human MANEA.

shADV-214892

Ad-h-MANEA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human MANEA (mannosidase endo-alpha). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ENDO; hEndo

Description (Vector)

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 (PubMed 15677381)).(supplied by OMIM, Sep 2008)

Gene ID

79694

Gene Name (Vector)

mannosidase endo-alpha

Gene Symbol

MANEA

HGNC ID

HGNC:21072

ORF Size (aa)

1386

ORF Size (bp)

1386 bp

RefSeq ID

NM_024641

RefSeq Synonyms

NM_024641, BC150199, BC146689, BC146671, BC137016, BC137014,

Species

human

UniGene ID

Hs.533323

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.