human LMNA shRNA silencing Adenovirus

Name 
Ad-h-LMNA-shRNA
Cat No 
shADV-214027
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human LMNA.

shADV-214027
Ad-h-LMNA-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human LMNA (lamin A/C). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C-like 1; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; prelamin-A/C; PRO1; renal carcinoma antigen NY-REN-32
Description (eCommerce)
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin A/C plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P02545].
Description (Vector)
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Gene ID
4000
Gene Name (eCommerce)
lamin A/C
Gene Name (Vector)
lamin A/C
Gene Symbol
LMNA
HGNC ID
HGNC:6636
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1995
ORF Size (bp)
1995 bp
Protein Name (eCommerce)
Prelamin-A/C
RefSeq ID
NM_170707
RefSeq Synonyms
NM_170708, NM_170707, NM_005572, NM_001282626, NM_001282625, NM_001282624, NM_001257374, BC033088, BC014507, BC003162, BC000511,
Research Areas
Aging,Apoptosis,Cardiac Development,Cardiology,Cardiomyopathy,Cardiovascular,CD95/FAS Signaling,Cell Cycle,Host-Virus Interactions,Hypoxia,Neurobiology,Neurodegeneration,Neurodevelopment,Signal Transduction,Spermatogenesis,TNF Signaling
Research Areas (Faceted)
cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,infectious_disease,neurobiology,signal_transduction
Species
human
Target Sentence
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin A/C plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P02545].
UniGene ID
Hs.594444
UniProt ID (eCommerce)
P02545

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.