human IWS1 shRNA silencing Adenovirus

Name 
Ad-h-IWS1-shRNA
Cat No 
shADV-212564
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human IWS1.

shADV-212564
Ad-h-IWS1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human IWS1 (IWS1, SUPT6H interacting protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
interacts with Spt6; IWS1 homolog; IWS1-like protein; protein IWS1 homolog
Description (eCommerce)
IWS1 homolog is a transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. IWS1 acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96ST2].
Gene ID
55677
Gene Name (eCommerce)
IWS1, SUPT6H interacting protein
Gene Name (Vector)
IWS1, SUPT6H interacting protein
Gene Symbol
IWS1
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2460
ORF Size (bp)
2460 bp
Protein Name (eCommerce)
Protein IWS1 homolog
RefSeq ID
NM_017969
RefSeq Synonyms
NM_017969, BC110537, BC110536, BC017012,
Research Areas
mRNA Processing,mRNA Splicing,mRNA transport,RNA Processing,RNA Transport,Transcription Factor/Regulator,Transport
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
IWS1 homolog is a transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. IWS1 acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96ST2].
UniGene ID
Hs.469879
UniProt ID (eCommerce)
Q96ST2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.