human ITSN1 shRNA silencing Adenovirus

Name 
Ad-h-ITSN1-shRNA
Cat No 
shADV-212559
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ITSN1.

shADV-212559
Ad-h-ITSN1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ITSN1 (intersectin 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
human intersectin-SH3 domain-containing protein SH3P17; intersectin 1 (SH3 domain protein); intersectin 1 short form variant 3; intersectin 1 short form variant, 11; intersectin short variant 12; intersectin-1; ITSN; SH3 domain-containing protein 1A; SH3D1A; SH3P17; Src homology 3 domain-containing protein
Description (eCommerce)
Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. Intersectin 1 may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q15811].
Description (Vector)
ITSN1 is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
Gene ID
6453
Gene Name (eCommerce)
intersectin 1
Gene Name (Vector)
intersectin 1
Gene Symbol
ITSN1
HGNC ID
HGNC:6183
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5151
ORF Size (bp)
5151 bp
Protein Name (eCommerce)
Intersectin-1
RefSeq ID
NM_003024
RefSeq Synonyms
NM_003024, NM_001331012, NM_001331011, NM_001331010, NM_001331009, NM_001331008, NM_001001132, BC117560, BC116186, BC116185,
Research Areas
Apoptosis,GPCR Signaling,Neurobiology,Neurodevelopment,NGF Signaling,Rho Signaling,Signal Transduction,Synapse
Research Areas (Faceted)
signal_transduction,cell_biology,neurobiology,developmental_biology,signal_transduction,neurobiology
Species
human
Target Sentence
Intersectin 1 is an adapter protein that may provide an indirect link between the endocytic membrane traffic and the actin assembly machinery. Intersectin 1 may regulate the formation of clathrin-coated vesicles. Isoform 1 could be involved in brain-specific synaptic vesicle recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q15811].
UniGene ID
Hs.160324
UniProt ID (eCommerce)
Q15811

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.