human IDH3G shRNA silencing Adenovirus

Name 
Ad-h-IDH3G-shRNA
Cat No 
shADV-211775
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human IDH3G.

shADV-211775
Ad-h-IDH3G-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human IDH3G (isocitrate dehydrogenase 3 (NAD(+)) gamma). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
H-IDHG
Description (Vector)
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.
Gene ID
3421
Gene Name (Vector)
isocitrate dehydrogenase 3 (NAD(+)) gamma
Gene Symbol
IDH3G
HGNC ID
HGNC:5386
ORF Size (aa)
1182
ORF Size (bp)
1182 bp
RefSeq ID
NM_174869
RefSeq Synonyms
NM_174869, NM_004135, BC001902, BC000933,
Species
human
UniGene ID
Hs.410197

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.