human GTF3C3 shRNA silencing Adenovirus

Name 
Ad-h-GTF3C3-shRNA
Cat No 
shADV-210655
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human GTF3C3.

shADV-210655
Ad-h-GTF3C3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human GTF3C3 (general transcription factor IIIC subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
general transcription factor 3C polypeptide 3; general transcription factor IIIC, polypeptide 3, 102kDa; TF3C-gamma; TFIIIC 102 kDa subunit; TFIIIC102; TFiiiC2-102; TFIIICgamma; Transcription factor IIIC 102 kDa subunit; transcription factor IIIC subunit gamma
Description (eCommerce)
General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.
Description (Vector)
GTF3C3 is part of the TFIIIC2 complex, which binds to the promoters of small nuclear and cytoplasmic RNA genes in order to recruit RNA polymerase III. The TFIIIC2 complex is composed of six subunits. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
9330
Gene Name (eCommerce)
general transcription factor IIIC subunit 3
Gene Name (Vector)
general transcription factor IIIC subunit 3
Gene Symbol
GTF3C3
HGNC ID
HGNC:4666
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2661
ORF Size (bp)
2661 bp
Protein Name (eCommerce)
General transcription factor 3C polypeptide 3
RefSeq ID
NM_012086
RefSeq Synonyms
NM_012086, NM_001206774, BC043347,
Research Areas
Gene Expression,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.
UniGene ID
Hs.470996
UniProt ID (eCommerce)
Q9Y5Q9

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.