human GCC2 shRNA silencing Adenovirus

Name 
Ad-h-GCC2-shRNA
Cat No 
shADV-209732
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human GCC2.

shADV-209732
Ad-h-GCC2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human GCC2 (GRIP and coiled-coil domain containing 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
72
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
185 kDa Golgi coiled-coil protein; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; GCC protein, 185-kD; GCC185; Golgi coiled-coil protein GCC185; GRIP and coiled-coil domain-containing protein 2; Ran-binding protein 2-like 4; RANBP2L4; REN53; renal carcinoma antigen NY-REN-53
Description (eCommerce)
185 kDa Golgi coiled-coil protein (GCC85) is a Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector, GCC85 is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. Golgin may also play a role in transport between the recycling endosomes and the Golgi. It is required for maintenance of the Golgi structure, and it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2. RanBP2L4, renal carcinoma antigen NY-REN-53, and KIAA0336 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q81WJ2].
Description (Vector)
GCC2 is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.
Gene ID
9648
Gene Name (eCommerce)
GRIP and coiled-coil domain containing 2
Gene Name (Vector)
GRIP and coiled-coil domain containing 2
Gene Symbol
GCC2
HGNC ID
HGNC:23218
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4752
ORF Size (bp)
4752 bp
Protein Name (eCommerce)
GRIP and coiled-coil domain-containing protein 2
RefSeq ID
NM_181453
RefSeq Synonyms
NM_181453, BC146789,
Research Areas
Bladder Cancer,Cancer,Golgi Apparatus,Lysosomal Transport,Transport
Research Areas (Faceted)
cancer,cell_biology
Species
human
Target Sentence
185 kDa Golgi coiled-coil protein (GCC85) is a Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector, GCC85 is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. Golgin may also play a role in transport between the recycling endosomes and the Golgi. It is required for maintenance of the Golgi structure, and it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2. RanBP2L4, renal carcinoma antigen NY-REN-53, and KIAA0336 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q81WJ2].
UniGene ID
Hs.436505
UniProt ID (eCommerce)
Q8IWJ2

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.