human GALT shRNA silencing Adenovirus

Name 
Ad-h-GALT-shRNA
Cat No 
shADV-209625
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human GALT.

shADV-209625
Ad-h-GALT-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human GALT (galactose-1-phosphate uridylyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
2592
Gene Name (Vector)
galactose-1-phosphate uridylyltransferase
Gene Symbol
GALT
HGNC ID
HGNC:4135
ORF Size (aa)
1140
ORF Size (bp)
1140 bp
RefSeq ID
NM_000155
RefSeq Synonyms
NM_001258332, NM_000155, BC015045,
Species
human
UniGene ID
Hs.522090

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.