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human G3BP1 shRNA silencing Adenovirus

Name 
Ad-h-G3BP1-shRNA
Cat No 
shADV-209475
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human G3BP1.

shADV-209475
Ad-h-G3BP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human G3BP1 (G3BP stress granule assembly factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; ras GTPase-activating protein-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein
Description (eCommerce)
Ras-GAP SH3 domain binding protein (G3BP1) contains an RNA recognition motif (RRM) and an N-terminal nuclear transport factor 2-like (NTF2-like) domain. It was first identified in a screen for proteins that bind the SH3 domain of the Ras GTPase activating protein (RasGAP). It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is primarily cytoplasmic but has also been localized to the nucleus. G3BP1 has been shown to be overexpressed in human cancers.
Description (Vector)
G3BP1 encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Gene ID
10146
Gene Name (eCommerce)
G3BP stress granule assembly factor 1
Gene Name (Vector)
G3BP stress granule assembly factor 1
Gene Symbol
G3BP1
HGNC ID
HGNC:30292
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1401
ORF Size (bp)
1401 bp
Protein Name (eCommerce)
Ras GTPase-activating protein-binding protein 1
RefSeq ID
NM_005754
RefSeq Synonyms
NM_198395, NM_005754, BC108278, BC006997,
Research Areas
Helicase Activity,RNA Binding,Signal Transduction
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
Ras-GAP SH3 domain binding protein (G3BP1) contains an RNA recognition motif (RRM) and an N-terminal nuclear transport factor 2-like (NTF2-like) domain. It was first identified in a screen for proteins that bind the SH3 domain of the Ras GTPase activating protein (RasGAP). It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is primarily cytoplasmic but has also been localized to the nucleus. G3BP1 has been shown to be overexpressed in human cancers.
UniGene ID
Hs.587054
UniProt ID (eCommerce)
Q13283

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.