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human EN1 shRNA silencing Adenovirus

Name 
Ad-h-EN1-shRNA
Cat No 
shADV-207899
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human EN1.

shADV-207899
Ad-h-EN1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human EN1 (engrailed homeobox 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Gene ID
2019
Gene Name (Vector)
engrailed homeobox 1
Gene Symbol
EN1
HGNC ID
HGNC:3342
ORF Size (aa)
1176
ORF Size (bp)
1176 bp
RefSeq ID
NM_001426
RefSeq Synonyms
NM_001426, BC111840,
Species
human
UniGene ID
Hs.271977

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.