human EIF5 shRNA silencing Adenovirus

Name 
Ad-h-EIF5-shRNA
Cat No 
shADV-207800
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human EIF5.

shADV-207800
Ad-h-EIF5-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human EIF5 (eukaryotic translation initiation factor 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
EIF-5; EIF-5A; eukaryotic translation initiation factor 5
Description (eCommerce)
Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.
Description (Vector)
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 (PubMed 8663286)).(supplied by OMIM, May 2010)
Gene ID
1983
Gene Name (eCommerce)
eukaryotic translation initiation factor 5
Gene Name (Vector)
eukaryotic translation initiation factor 5
Gene Symbol
EIF5
HGNC ID
HGNC:3299
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1296
ORF Size (bp)
1296 bp
Protein Name (eCommerce)
Eukaryotic translation initiation factor 5
RefSeq ID
NM_001969
RefSeq Synonyms
NM_183004, NM_001969, BC032866, BC007728,
Research Areas
Gene Expression,Metabolism/Metabolic Process,RNA Processing,RNA Transport,Translational Control
Research Areas (Faceted)
cell_biology,genetics,transcription_translation,metabolism
Species
human
Target Sentence
Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.
UniGene ID
Hs.433702
UniProt ID (eCommerce)
P55010

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.