human EEF2 shRNA silencing Adenovirus

Name 
Ad-h-EEF2-shRNA
Cat No 
shADV-207601
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human EEF2.

shADV-207601
Ad-h-EEF2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human EEF2 (eukaryotic translation elongation factor 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
EEF-2; EF2; EF-2; elongation factor 2; epididymis secretory sperm binding protein; polypeptidyl-tRNA translocase; SCA26
Description (eCommerce)
Eukaryotic translation elongation factor 2 (eEF2) is a non-ribosomal protein that mediates ribosomal translocation and catalyzes peptide elongation in the presence of GTP. eEF2 is a substrate of the eEF2 kinase. Phosphorylation of eEF2 in response to increased energy demand or reduction in supply inhibits eEF2 activity for the conservation of energy.
Description (Vector)
EEF2 encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Gene ID
1938
Gene Name (eCommerce)
eukaryotic translation elongation factor 2
Gene Name (Vector)
eukaryotic translation elongation factor 2
Gene Symbol
EEF2
HGNC ID
HGNC:3214
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2577
ORF Size (bp)
2577 bp
Protein Name (eCommerce)
Elongation factor 2
RefSeq ID
NM_001961
RefSeq Synonyms
NM_001961, BC136313, BC126259,
Research Areas
BDNF Signaling,Gene Expression,Metabolism/Metabolic Process,mTOR Signaling,Neurobiology,Neurodegeneration,Signal Transduction,Translational Control
Research Areas (Faceted)
cell_biology,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Eukaryotic translation elongation factor 2 (eEF2) is a non-ribosomal protein that mediates ribosomal translocation and catalyzes peptide elongation in the presence of GTP. eEF2 is a substrate of the eEF2 kinase. Phosphorylation of eEF2 in response to increased energy demand or reduction in supply inhibits eEF2 activity for the conservation of energy.
UniGene ID
Hs.515070
UniProt ID (eCommerce)
P13639

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.