human DAZAP1 shRNA silencing Adenovirus

Name 
Ad-h-DAZAP1-shRNA
Cat No 
shADV-206611
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human DAZAP1.

shADV-206611
Ad-h-DAZAP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DAZAP1 (DAZ associated protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DAZ-associated protein 1; deleted in azoospermia-associated protein 1; testicular tissue protein Li 50
Description (eCommerce)
Deleted in azoospermia-associated protein 1 (DAZAP1) is an RNA-binding protein, which may be required during spermatogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96EP5].
Description (Vector)
In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
Gene ID
26528
Gene Name (eCommerce)
DAZ associated protein 1
Gene Name (Vector)
DAZ associated protein 1
Gene Symbol
DAZAP1
HGNC ID
HGNC:2683
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1224
ORF Size (bp)
1224 bp
Protein Name (eCommerce)
DAZ-associated protein 1
RefSeq ID
NM_018959
RefSeq Synonyms
NM_170711, NM_018959, BC012062,
Research Areas
RNA Binding,RNA Processing,Spermatogenesis
Research Areas (Faceted)
cell_biology,developmental_biology,genetics,transcription_translation
Species
human
Target Sentence
Deleted in azoospermia-associated protein 1 (DAZAP1) is an RNA-binding protein, which may be required during spermatogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96EP5].
UniGene ID
Hs.222510
UniProt ID (eCommerce)
Q96EP5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.