human DARS shRNA silencing Adenovirus

Name 
Ad-h-DARS-shRNA
Cat No 
shADV-206603
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human DARS.

shADV-206603
Ad-h-DARS-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DARS (aspartyl-tRNA synthetase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
aspartate tRNA ligase 1, cytoplasmic; aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; aspartyl-tRNA synthetase, cytoplasmic; aspRS; cell proliferation-inducing gene 40 protein; HBSL; testicular tissue protein Li 192
Description (eCommerce)
Aspartyl-tRNA Synthetase/DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14868].
Description (Vector)
Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
Gene ID
1615
Gene Name (eCommerce)
aspartyl-tRNA synthetase
Gene Name (Vector)
aspartyl-tRNA synthetase
Gene Symbol
DARS
HGNC ID
HGNC:2678
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1506
ORF Size (bp)
1506 bp
Protein Name (eCommerce)
Aspartate--tRNA ligase, cytoplasmic
RefSeq ID
NM_001349
RefSeq Synonyms
NM_001349, NM_001293312, BC107749, BC000629,
Research Areas
Gene Expression,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Aspartyl-tRNA Synthetase/DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14868].
UniGene ID
Hs.503787
UniProt ID (eCommerce)
P14868

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.