human CH25H shRNA silencing Adenovirus

Name 
Ad-h-CH25H-shRNA
Cat No 
shADV-205084
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human CH25H.

shADV-205084
Ad-h-CH25H-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CH25H (cholesterol 25-hydroxylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C25H
Description (Vector)
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Gene ID
9023
Gene Name (Vector)
cholesterol 25-hydroxylase
Gene Symbol
CH25H
HGNC ID
HGNC:1907
ORF Size (aa)
819
RefSeq ID
NM_003956
RefSeq Synonyms
NM_003956, BC072430, BC017843,
Species
human
UniGene ID
Hs.47357

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.