human CENPF shRNA silencing Adenovirus

Name 
Ad-h-CENPF-shRNA
Cat No 
shADV-204955
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human CENPF.

shADV-204955
Ad-h-CENPF-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human CENPF (centromere protein F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
Description (eCommerce)
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
Description (Vector)
CENPF encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
Gene ID
1063
Gene Name (eCommerce)
centromere protein F
Gene Name (Vector)
centromere protein F
Gene Symbol
CENPF
HGNC ID
HGNC:1857
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
9345
ORF Size (bp)
9345 bp
Protein Name (eCommerce)
Centromere protein F
RefSeq ID
NM_016343
RefSeq Synonyms
NM_016343, BC172232,
Research Areas
Cancer,Cell Cycle,Cell Division,Cytoskeleton,DNA Replication,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
UniGene ID
Hs.497741
UniProt ID (eCommerce)
P49454

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.