human C10ORF2 shRNA silencing Adenovirus

Name 
Ad-h-C10ORF2-shRNA
Cat No 
shADV-202407
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human TWNK.

shADV-202407
Ad-h-C10ORF2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TWNK (twinkle mtDNA helicase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATXN8; C10orf2; IOSCA; MTDPS7; PEO; PEO1; PEOA3; PRLTS5; SANDO; SCA8; TWINL
Description (Vector)
C10orf2 encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
56652
Gene Name (Vector)
twinkle mtDNA helicase
Gene Symbol
TWNK
HGNC ID
HGNC:1160
ORF Size (aa)
1521
ORF Size (bp)
1521 bp
RefSeq ID
NM_021830
RefSeq Synonyms
NM_021830, NM_001163814, NM_001163813, NM_001163812, BC033762,
Species
human
UniGene ID
Hs.22678

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.