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human BYSL shRNA silencing Adenovirus

Name 
Ad-h-BYSL-shRNA
Cat No 
shADV-202368
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human BYSL.

shADV-202368
Ad-h-BYSL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human BYSL (bystin like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
by the ribosomal protein s6 gene, drosophila, homolog-like; BYSTIN; Enp1
Description (eCommerce)
Bystin is required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. it may be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13895].
Description (Vector)
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.
Gene ID
705
Gene Name (eCommerce)
bystin like
Gene Name (Vector)
bystin like
Gene Symbol
BYSL
HGNC ID
HGNC:1157
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1314
ORF Size (bp)
1314 bp
Protein Name (eCommerce)
Bystin
RefSeq ID
NM_004053
RefSeq Synonyms
NM_004053, BC062627, BC050645, BC007340,
Research Areas
Cell Biology,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Bystin is required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. it may be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13895].
UniGene ID
Hs.106880
UniProt ID (eCommerce)
Q13895

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.