human ATP6V1G1 shRNA silencing Adenovirus

Name
Ad-h-ATP6V1G1-shRNA

Cat No
shADV-201771

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human ATP6V1G1.

shADV-201771

Ad-h-ATP6V1G1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATP6V1G1 (ATPase H+ transporting V1 subunit G1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ATP6G; ATP6G1; ATP6GL; ATP6J; Vma10

Description (Vector)

ATP6V1G1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized.

Gene ID

9550

Gene Name (Vector)

ATPase H+ transporting V1 subunit G1

Gene Symbol

ATP6V1G1

HGNC ID

HGNC:864

ORF Size (aa)

357

ORF Size (bp)

357 bp

RefSeq ID

NM_004888

RefSeq Synonyms

NM_004888, BC008452, BC003564,

Species

human

UniGene ID

Hs.388654

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.