human ATP6V0B shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human ATP6V0B.
shADV-201746
Ad-h-ATP6V0B-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human ATP6V0B (ATPase H+ transporting V0 subunit b). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
ATP6F; HATPL; VMA16
Description (Vector)
ATP6V0B encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
Gene ID
533
Gene Name (Vector)
ATPase H+ transporting V0 subunit b
Gene Symbol
ATP6V0B
HGNC ID
HGNC:861
ORF Size (aa)
618
ORF Size (bp)
618 bp
RefSeq ID
NM_004047
RefSeq Synonyms
NM_004047, NM_001294333, NM_001039457, BC005876, BC000423,
Species
human
UniGene ID
Hs.596514
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.