human ASAH1 shRNA silencing Adenovirus

Name
Ad-h-ASAH1-shRNA

Cat No
shADV-201505

Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Human ASAH1.

shADV-201505

Ad-h-ASAH1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ASAH1 (N-acylsphingosine amidohydrolase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

AC; ACDase; ASAH; PHP; PHP32; SMAPME

Description (Vector)

ASAH1 encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.

Gene ID

427

Gene Name (Vector)

N-acylsphingosine amidohydrolase 1

Gene Symbol

ASAH1

HGNC ID

HGNC:735

ORF Size (aa)

1170

ORF Size (bp)

1170 bp

RefSeq ID

NM_004315

RefSeq Synonyms

NM_177924, NM_004315, NM_001127505, BC016828, BC016481,

Species

human

UniGene ID

Hs.633993

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.