human APTX shRNA silencing Adenovirus

Name 
Ad-h-APTX-shRNA
Cat No 
shADV-201227
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human APTX.

shADV-201227
Ad-h-APTX-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human APTX (aprataxin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
53
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AOA; AOA1; aprataxin; AXA1; EAOH; EOAHA; FHA-HIT; forkhead-associated domain histidine triad-like protein
Description (eCommerce)
The Aprataxin gene was identified by linkage analysis as the defective gene in the neurogenerative disorder ataxia with oculomotor apraxia type 1 (AOA1) which is characterized by cerebellar ataxia, peripheral neuropathy, and hypoalbuminemia. Aprataxin functions in the DNA damage response and is involved in single-strand and double-strand break repair, and base excision repair.
Description (Vector)
APTX encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Gene ID
54840
Gene Name (eCommerce)
aprataxin
Gene Name (Vector)
aprataxin
Gene Symbol
APTX
HGNC ID
HGNC:15984
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
765
ORF Size (bp)
765 bp
Protein Name (eCommerce)
Aprataxin
RefSeq ID
NM_001195254
RefSeq Synonyms
NM_175073, NM_175069, NM_001195254, NM_001195252, NM_001195251, NM_001195250, NM_001195249, NM_001195248, BC104881, BC032650, BC001628,
Research Areas
Apoptosis,DNA Damage/Repair,Neurobiology,Neurodegeneration,RNA Binding,Zinc-finger
Research Areas (Faceted)
cell_biology,genetics,transcription_translation,neurobiology,genetics,cell_biology
Species
human
Target Sentence
The Aprataxin gene was identified by linkage analysis as the defective gene in the neurogenerative disorder ataxia with oculomotor apraxia type 1 (AOA1) which is characterized by cerebellar ataxia, peripheral neuropathy, and hypoalbuminemia. Aprataxin functions in the DNA damage response and is involved in single-strand and double-strand break repair, and base excision repair.
UniGene ID
Hs.20158
UniProt ID (eCommerce)
Q7Z2E3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.