human ANAPC5 shRNA silencing Adenovirus

Name 
Ad-h-ANAPC5-shRNA
Cat No 
shADV-200901
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human ANAPC5.

shADV-200901
Ad-h-ANAPC5-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ANAPC5 (anaphase promoting complex subunit 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
anaphase-promoting complex subunit 5; APC5; cyclosome subunit 5
Description (eCommerce)
APC5 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. APC5 may serve a scaffolding role that connects the enzymatic core APC subunits with the regulatory APC subunits.
Description (Vector)
ANAPC5 encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
Gene ID
51433
Gene Name (eCommerce)
anaphase promoting complex subunit 5
Gene Name (Vector)
anaphase promoting complex subunit 5
Gene Symbol
ANAPC5
HGNC ID
HGNC:15713
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2268
ORF Size (bp)
2268 bp
Protein Name (eCommerce)
Anaphase-promoting complex subunit 5
RefSeq ID
NM_016237
RefSeq Synonyms
NM_016237, NM_001330489, NM_001137559, BC006301, BC001950, BC001081,
Research Areas
Antigen Processing and Presentation,Cell Cycle,Cell Division,Host-Virus Interactions,Immunology,Meiosis,Signal Transduction,TGF-beta Signaling,Ubiquitination
Research Areas (Faceted)
cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
APC5 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. APC5 may serve a scaffolding role that connects the enzymatic core APC subunits with the regulatory APC subunits.
UniGene ID
Hs.7101
UniProt ID (eCommerce)
Q9UJX4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.