human ADD2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human ADD2.
shADV-200443
Ad-h-ADD2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human ADD2 (adducin 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
ADDB; adducin 2 (beta); beta-adducin; erythrocyte adducin subunit beta
Description (eCommerce)
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Heterodimers consisting of alpha and gamma subunits have also been described [taken from NCBI Entrez Gene (Gene ID: 119)].
Description (Vector)
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.
Gene ID
119
Gene Name (eCommerce)
adducin 2
Gene Name (Vector)
adducin 2
Gene Symbol
ADD2
HGNC ID
HGNC:244
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2181
ORF Size (bp)
2181 bp
Protein Name (eCommerce)
Beta-adducin
RefSeq ID
NM_017488
RefSeq Synonyms
NM_017488, NM_017482, NM_001617, NM_001185055, NM_001185054, BC065525, BC041666,
Research Areas
Cytoskeleton
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Heterodimers consisting of alpha and gamma subunits have also been described [taken from NCBI Entrez Gene (Gene ID: 119)].
UniGene ID
Hs.188528
UniProt ID (eCommerce)
P35612
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.