rat NAE1 shRNA silencing AAV

Name
AAV-r-NAE1-shRNA

Cat No
shAAV-295076

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Nae1.

shAAV-295076

AAV-r-NAE1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Nae1 (NEDD8 activating enzyme E1 subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Appbp1; RGD1563180

Description (Vector)

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Gene ID

84019

Gene Name (Vector)

NEDD8 activating enzyme E1 subunit 1

Gene Symbol

Nae1

ORF Size (aa)

1605

ORF Size (bp)

1605 bp

RefSeq ID

NM_032072

RefSeq Synonyms

NM_032072, BC127481,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.