rat NEDD4L shRNA silencing AAV

Name
AAV-r-NEDD4L-shRNA

Cat No
shAAV-293843

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Nedd4l.

shAAV-293843

AAV-r-NEDD4L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Nedd4l (neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Nedd4-2

Description (Vector)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

Gene ID

291553

Gene Name (Vector)

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Gene Symbol

Nedd4l

ORF Size (aa)

1323

ORF Size (bp)

1323 bp

RefSeq ID

NM_001008300

RefSeq Synonyms

NM_001008300, BC086371,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.