rat KLHL12 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Rat Klhl12.
shAAV-292288
AAV-r-KLHL12-shRNA
Ready-to-use AAV expressing shRNA for silencing of Rat Klhl12 (kelch-like family member 12). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
C3ip1
Description (Vector)
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.
Gene ID
266772
Gene Name (Vector)
kelch-like family member 12
Gene Symbol
Klhl12
ORF Size (aa)
1707
ORF Size (bp)
1707 bp
RefSeq ID
NM_153730
RefSeq Synonyms
NM_153730, BC086983,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.