rat SPHK1 shRNA silencing AAV

Name
AAV-r-SPHK1-shRNA

Cat No
shAAV-291589

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Sphk1.

shAAV-291589

AAV-r-SPHK1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Sphk1 (sphingosine kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Gene ID

170897

Gene Name (Vector)

sphingosine kinase 1

Gene Symbol

Sphk1

ORF Size (aa)

1152

ORF Size (bp)

1152 bp

RefSeq ID

NM_001270808

RefSeq Synonyms

NM_133386, NM_001270811, NM_001270810, NM_001270809, NM_001270808, NM_001270807, BC081738,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.