rat SF3B4 shRNA silencing AAV

Name
AAV-r-SF3B4-shRNA

Cat No
shAAV-291125

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Rat Sf3b4.

shAAV-291125

AAV-r-SF3B4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Sf3b4 (splicing factor 3b, subunit 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Gene ID

295270

Gene Name (Vector)

splicing factor 3b, subunit 4

Gene Symbol

Sf3b4

ORF Size (aa)

1275

ORF Size (bp)

1275 bp

RefSeq ID

NM_001011951

RefSeq Synonyms

NM_001011951, BC078997,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.