rat GPR177 shRNA silencing AAV

Name
AAV-r-GPR177-shRNA

Cat No
shAAV-290514

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Rat Wls.

shAAV-290514

AAV-r-GPR177-shRNA

Ready-to-use AAV expressing shRNA for silencing of Rat Wls (wntless Wnt ligand secretion mediator). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Gpr177

Description (Vector)

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).

Gene ID

362065

Gene Name (Vector)

wntless Wnt ligand secretion mediator

Gene Symbol

Wls

ORF Size (aa)

1626

ORF Size (bp)

1626 bp

RefSeq ID

NM_199408

RefSeq Synonyms

NM_199408, NM_001085353, BC062391,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.