mouse SPAG5 shRNA silencing AAV

Name 
AAV-m-SPAG5-shRNA
Cat No 
shAAV-281125
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Spag5.

shAAV-281125
AAV-m-SPAG5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Spag5 (sperm associated antigen 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AI874642; D11Bhm180e; Deepest; MAP126; Mastrin; S17
Description (Vector)
Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.
Gene ID
54141
Gene Name (Vector)
sperm associated antigen 5
Gene Symbol
Spag5
ORF Size (aa)
3498
ORF Size (bp)
3498 bp
RefSeq ID
NM_017407
RefSeq Synonyms
NM_017407, BC052672,
Species
mouse
UniGene ID
Mm.24250

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.