mouse MASTL shRNA silencing AAV

Name
AAV-m-MASTL-shRNA

Cat No
shAAV-280876

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Mastl.

shAAV-280876

AAV-m-MASTL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Mastl (microtubule associated serine/threonine kinase-like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2700091H24Rik; C88295; GW; GWL; MAST-L; THC2

Description (Vector)

Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

Gene ID

67121

Gene Name (Vector)

microtubule associated serine/threonine kinase-like

Gene Symbol

Mastl

ORF Size (aa)

2598

ORF Size (bp)

2598 bp

RefSeq ID

NM_025979

RefSeq Synonyms

NM_025979, BC103779,

Species

mouse

UniGene ID

Mm.253721

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.