mouse WIBG shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Pym1.
shAAV-276290
AAV-m-WIBG-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Pym1 (PYM homolog 1, exon junction complex associated factor). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
A030010B05Rik; Pym; Wibg
Description (Vector)
Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.
Gene ID
78428
Gene Name (Vector)
PYM homolog 1, exon junction complex associated factor
Gene Symbol
Pym1
ORF Size (aa)
609
RefSeq ID
NM_030100
RefSeq Synonyms
NM_030100, NM_001253705, NM_001253704, NM_001170869, BC049647,
Species
mouse
UniGene ID
Mm.27358
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.